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In this press release, we announce the launch of the PhotonFit analysis software, a streamlined software platform designed to remove long-standing technical barriers to smFRET, FCS, and FCCS data processing.
Discover how solution-phase single-molecule techniques (smFRET, FCS, FCCS) bridge structural biology with kinetic mechanisms across nutrient sensing, viral replication, and therapeutic antibody targeting.
Learn how researchers used a universal protein FRET ladder to calibrate FRET measurements across EI-FLEX, FLIM-FRET and flow cytometry.
In this second celebratory article, we explore how smFRET has revolutionised our understanding of biomolecular mechanisms and how this is now being applied to modern therapeutics.
Hear from Alex Payne-Dwyer, Senior Technical Specialist at the University of York, who discusses his experiences of running the EI-FLEX in a core facility.
Bringing smFRET, FCS, and FCCS out of the specialist dark room, the EI-FLEX system is democratising advanced biophysics in core facilities by combining single-molecule resolution with simple, code-free workflows that maximise institutional utility.
As smFRET turns 30, trace its evolution from a ground-breaking physics concept into the democratised, high-throughput benchtop tool that is transforming the life sciences and modern drug discovery.
In-cell labelling for smFRET and direct analysis of cell lysates have a wide range of benefits. See how this was applied to generate a universal protein ladder for FRET.
Wondering whether your tethered data is revealing true biological insights or immobilisation artefacts? Explore why combining kinetics on the surface with solution-phase single-molecule validation is essential to avoid misleading data.
Curious about how to label samples for smFRET experiments? Explore practical considerations and recommended methods in this technical note.
Discover everything you need to understand fluorescence correlation spectroscopy (FCS) and fluorescence cross-correlation spectroscopy (FCCS) and use it to advance your research. Download the handbook now.
Explore the 'messy middle' of biophysics, where cell lysates and serum are combined with single-molecule sensitivity to preserve biological context.

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