The EI-FLEX system is an easy-to-use, cost-effective, benchtop instrument capable of two-colour, single-molecule fluorescence detection in solution, with two key modes:
Single-molecule Forster Resonance Energy Transfer (smFRET Mode).
Fluorescence Correlation Spectroscopy (FCS Mode)
Equipped with advanced software to provide real-time data visualisations and complex data analysis at the push of a button. The EI-FLEX is an affordable platform technology for making discoveries in biophysics, life sciences, drug development and medical diagnostics.Contact us
Go beyond the capabilities of traditional assay methods and plate readers with single-molecule sensitivity.
Make precise distance measurements in the 3- 10 nm range using our smRULER calibration features.
Perform accurate studies of chemical, biological and physical processes at low (picomolar) concentrations.
Measure the interactions, complexing and kinetics of DNA, proteins, antibodies, enzymes and kinases.
Easy-to-use software with real-time graphing and in-built analysis tools help you accelerate the assay validation process.
Cloud connectivity makes your collaborations agile, enabling results sharing within research groups.
Exciting Instruments Limited is a joint venture, built in Sheffield, stemming from the software brains of Bulldozer Limited and the single-molecule expertise of Dr Timothy Craggs. We are a true partnership of science and software, with a mission to help you simplify the process of making discoveries in biophysics, life sciences, drug discovery and medical diagnostics, through the use of cloud connectivity and real-time data visualisation.
Founder and CSO
Tim is an internationally renowned expert in the development and application of single-molecule FRET methods and in the detailed biophysical characterisation of protein folding, and protein-nucleic acid interactions. As a champion for open science and accessibility, Tim founded Exciting Instruments in order to bring his team's developments in single-molecule spectroscopy to the broadest possible user base.
Rob leads software development, which forms the backbone of each of Exciting Instruments technology offerings. As a Director of Bulldozer Limited, and a lead software engineer at Razor Limited, Rob has built an extensive portfolio of successful grass-roots technology companies that have generated fast growth and unrivalled software functionality through his skill at taking ideas and making them a reality.
Chris Sellars is a specialist in providing intelligent, proactive and candid advice to technology companies, and brings a pragmatic approach when making the Exciting Instruments vision a reality. Chris’ background in corporate finance provides the solid foundation which continues to drive Exciting Instruments expansion.
The EI-FLEX system does not require an optical table and our online diagnostic tools will ensure repeatable performance.
The EI-FLEX system is a Class 1 laser product and may be used in a standard lab without specific laser training.
The EI-FLEX software is easy to use. Our cloud-based data analysis and graphing tools are making single molecule discoveries, at the push of a button, accessible to all users. Raw data downloads are available in HDF5 format to provide compatibility with all down-stream, data analysis software.
The EI-FLEX system is built on the vision of Dr Timothy Craggs and the Exciting Instruments team to create an affordable, bench-top smFRET/FCS system capable of meeting the exacting needs and build standards of single molecule specialists, whilst maintaining the ease-of-use and accessibility suitable for the broadest range of academic and industrial users. The EI-FLEX range is designed to grow with your lab, your research and your business. Please contact us to find out more about the EI-FLEX range and how Exciting Instruments can support your laboratory and your research.
Single-molecule FRET is a powerful tool for the measurement of inter and intramolecular distances; however, it requires the determination of absolute FRET efficiencies. Our smRULER feature utilises ALEX (alternating laser excitation) combined with our smRULER calibration standards and straight-forward calibration protocols to overcome these issues and make absolute distance measurements simpler and more accurate! smRULER is designed to give you greater confidence in uncovering molecule conformations and in assigning conformations to different structural states.
A recent worldwide benchmarking study (Nature Methods 2018) has shown these methods for absolute FRET determination to be both precise and accurate.
Our advanced software for the EI-FLEX system utilises cloud connectivity to perform complex data analysis in real-time, allowing you to visualise your data as it grows, helping to support simple and fast assay validations which accelerate the decision-making process.
smFRET measurements typically take around 30 minutes and FCS measurements around 1 minute. Our software allows you to view experiment progress in real-time, speeding up the discovery process.
The EI-FLEX system is compatible with a broad range of commercial probes which are suitable for traditional ALEX FRET measurements including Cy™, Atto-tec, Alexa Fluor, Janelia Fluor®, Fluorescent Proteins, CLIP-Cell™ and SNAP-Cell® fluorescent probes. We are constantly expanding the list of recommended probes, so please contact us for a list of our currently recommended probes for use with our dual excitation laser system.
We are currently offering the following optional add-ons for the EI-FLEX system:
Expanding the EI-FLEX system with features that will help you grow your research and your business is core to our vision. Please get-in touch and help us to do this.