EI-FLEX
See what really matters with true single-molecule sensitivity
Our essential, cost-effective instrument for every lab bench
For bringing your static structures to life seeing transient intermediates defining binding mechanisms expanding core facility ROI
The EI-FLEX system brings a biophysics professor into any lab with one simple, confocal benchtop solution that rapidly reveals physiologically-relevant behaviour without immobilising targets or requiring large sample volumes, all at single-molecule precision.
Single-molecule FRET (smFRET), fluorescence correlation spectroscopy (FCS) and fluorescence cross-correlation spectroscopy have always held extraordinary potential – the ability to watch molecular motion, reveal hidden intermediates, and uncover binding mechanisms that other techniques might not capture. What has been missing is access. By bringing these techniques into a compact, intuitive benchtop instrument, we’re opening this capability to every lab, not just the specialists.
You no longer need a dark room, a custom-built setup, or a team of experts. You simply need curiosity, a question, and a place on your bench. Because when powerful technology becomes accessible, scientific progress accelerates.
Looking for our new upgradable, automated system? Check out the EI-FLEX Pro here.
Real-time single-molecule confidence
Live time trace and histograms with background estimation and occupancy so you can verify true single-molecule acquisition from the first run.
Benchtop simplicity with no specialist setup
Ambient-light operation,
no optical table,
no laser lab,
and easy to perform manual calibration and acquisition.
Your professor in a box
Background correction, burst searching, and distance calculations via supplied protocols. Designed for experts and beginners alike.
Grant support
Optional provision of data or wording to help you get the best outcome.
Trusted by
Support your experiments from acquisition
through to analysis
Support your experiments from acquisition
through to analysis
Key applications explorer
Select an application to discover how freely diffusing, single-molecule methods on the EI-FLEX system can transform your research. View more applications here.
What our customers say
‘It has opened up new avenues of research because of the combined FCS, FCCS and single-molecule FRET capabilities. The high performance, stability and precision of the EI-FLEX instrument have really been a game-changer for experiments where sensitivity to picomolar concentration of biomolecules really matters.’
“Solution-based measurements, like those taken using the EI-FLEX, avoid surface tethering, which can introduce artefacts, especially for proteins that don’t behave well near hydrophobic surfaces. Keeping proteins in solution lets you observe their native conformation, and it’s much easier experimentally: you label, dilute, and measure, often getting results in minutes rather than days or weeks with TIRF-based systems.
“What sets the EI-FLEX apart is its versatility - it's our go-to for probing kinetics and conformational changes that the other systems can't resolve. This opens up a range of research for us that was previously impossible.”
What our customers say
‘It has opened up new avenues of research because of the combined FCS, FCCS and single-molecule FRET capabilities. The high performance, stability and precision of the EI-FLEX instrument have really been a game-changer for experiments where sensitivity to picomolar concentration of biomolecules really matters.’
“Solution-based measurements, like those taken using the EI-FLEX, avoid surface tethering, which can introduce artefacts, especially for proteins that don’t behave well near hydrophobic surfaces. Keeping proteins in solution lets you observe their native conformation, and it’s much easier experimentally: you label, dilute, and measure, often getting results in minutes rather than days or weeks with TIRF-based systems.
“What sets the EI-FLEX apart is its versatility - it's our go-to for probing kinetics and conformational changes that the other systems can't resolve. This opens up a range of research for us that was previously impossible.”
What our customers say
‘It has opened up new avenues of research because of the combined FCS, FCCS and single-molecule FRET capabilities. The high performance, stability and precision of the EI-FLEX instrument have really been a game-changer for experiments where sensitivity to picomolar concentration of biomolecules really matters.’
“Solution-based measurements, like those taken using the EI-FLEX, avoid surface tethering, which can introduce artefacts, especially for proteins that don’t behave well near hydrophobic surfaces. Keeping proteins in solution lets you observe their native conformation, and it’s much easier experimentally: you label, dilute, and measure, often getting results in minutes rather than days or weeks with TIRF-based systems.
“What sets the EI-FLEX apart is its versatility - it's our go-to for probing kinetics and conformational changes that the other systems can't resolve. This opens up a range of research for us that was previously impossible.”