Awarded the Rudolf-Kaiser-Preis in 2011 for his groundbreaking work on molecular machines and the chaperone protein Hsp90, Prof. Hugel exemplifies how single-molecule methods are transforming our understanding of biological complexity.
In this episode of Exciting Seminars, he discusses:
Beyond two colours: Overcoming the one-dimensional limitations of conventional smFRET by introducing a third fluorophore and building fluorophore networks for richer, multidimensional insights
Dynamic structures revealed: Measuring correlations, cooperativity, and allostery to uncover structural ensembles and dynamic conformations across a wide range of timescales—from microseconds to hours
In-cell biophysics: Extending smFRET into living cells to explore protein dynamics and phase separation phenomena
Research vision: Insights from the collaborative research centre Dynamic Organisation of Cellular Protein Machineries (SFB 1381) and the clusters of excellence livMatS and CiPSM, where Prof. Hugel continues to push the boundaries of single-molecule analysis
