In traditional drug discovery, we have long been settling for the average. In this article, we explore how single-molecule clarity de-risks biopharma pipelines, targets undruggable IDPs, and powers lab-in-the-loop AI discovery.
Discover how smFRET on the EI-FLEX reveals pH-driven DNA triplex nanoswitching, identifying the pH values at which full switching occurs, or heterogeneous populations are present.
In this application note, we explore how smFRET was used on the EI-FLEX to uncover two additional conformational states in the bacterial helicase Rep, expanding on previously defined structures.
Explore how smFRET and FCS can reveal the mechanism of action of two SARS-CoV-2 RNA-dependent RNA polymerase (RdRp) inhibitors using a doubly labelled RNA hairpin as a reporter for RNA extension.
To ensure calculated distances are accurate and relate to the physical positions of the labelled biomolecules of interest, we discuss the importance of data correction and additional modelling in this technical note.
Discover how EI-FLEX smFRET reveals pH-driven DNA triplex nanoswitching and confirms that dynamic DNA machines retain fast, reversible conformational control even when densely immobilised on electronic surfaces.
In this application note, we demonstrate how fluorescence correlation spectroscopy (FCS) can be performed using the EI-FLEX to assess antibody-antigen binding affinities and generate Kd values.
FCCS is an ideal technique for direct quantification of ternary complex formation. See how the EI-FLEX Pro was used to rapidly generate hook plots and derive time-resolved kinetics in a model DNA oligo system.
Discover how smFRET can capture conformational dynamics on the EI-FLEX Pro using a DNA hairpin model system, measuring the influence of salt on opening and closing rates.
