Curious to see smFRET data on ssDNA? Here, we show how the EI-FLEX confirmed DNA wrapping around replication protein A, providing consensus on previously conflicting structural data.
Discover how smFRET performed using the EI-FLEX resolved the conformational changes of a highly dynamic heterodimeric protein, Rag GTPase, that binds nucleotides and the serine/threonine protein kinase complex mTORC1.
In this second celebratory article, we explore how smFRET has revolutionised our understanding of biomolecular mechanisms and how this is now being applied to modern therapeutics.
Hear from Alex Payne-Dwyer, Senior Technical Specialist at the University of York, who discusses his experiences of running the EI-FLEX in a core facility.
Bringing smFRET, FCS, and FCCS out of the specialist dark room, the EI-FLEX system is democratising advanced biophysics in core facilities by combining single-molecule resolution with simple, code-free workflows that maximise institutional utility.
As smFRET turns 30, trace its evolution from a ground-breaking physics concept into the democratised, high-throughput benchtop tool that is transforming the life sciences and modern drug discovery.
Discover a simple, purification-free method to track real-time protein production and quantification by coupling cell-free protein synthesis with an FCS time course over several hours.
See how researchers used cryo-EM and smFRET to uncover how dynamic RNA structures within flavivirus genomes guide assembly of the viral replication machinery.
Discover how researchers used the EI-FLEX to track and characterise protein expression in real time, showcasing the power of FCS for rapid, purification-free protein analysis.
In-cell labelling for smFRET and direct analysis of cell lysates have a wide range of benefits. See how this was applied to generate a universal protein ladder for FRET.
Wondering whether your tethered data is revealing true biological insights or immobilisation artefacts? Explore why combining kinetics on the surface with solution-phase single-molecule validation is essential to avoid misleading data.
