Mastering
single-molecule FRET:
A practical handbook for researchers
Understand the fundamentals of smFRET and explore its potential in your own research​
Discover everything you need to understand single-molecule FRET and use it to advance your research
This practical smFRET handbook brings together the core principles, experimental workflows, and analytical strategies that underpin modern single-molecule F̦rster Resonance Energy Transfer (smFRET). Designed for researchers at every stage Рfrom newcomers to seasoned practitioners Рit distils the essential knowledge required to generate high-quality smFRET data and interpret it with confidence.
Inside, you’ll find:
Clear, accessible explanations of key concepts such as FRET efficiency, stoichiometry, ALEX, burst analysis, and accurate FRET correction
Step-by-step descriptions of how a confocal smFRET instrument works, how single molecules are detected, and how to ensure true single-molecule sensitivity in practice
Illustrative examples and case studies showing how smFRET resolves structural heterogeneity, conformational dynamics, and nanoscale distance changes in biomolecules
Comparisons to complementary single-molecule and structural biology techniques, helping you understand when – and why – smFRET is the method of choice
Insights into powerful analytical approaches, including E–S plotting, burst search strategies, and correction-factor estimation for high-precision distance measurements
Download this guide if you want to:
✓ introduce a simple, solution-based method into your existing workflows that rapidly resolves conformational changes at the single molecule level
✓ complement existing static workflows with dynamic data
✓ unlock deeper functional insights and novel biological questions
smFRET explained in 4 minutes
Watch the essentials before downloading the handbook 👇
What questions does the handbook answer?
- What is FRET?
- What are smFRET measurements?
- What is FRET efficiency and stoichiometry?
- What is Alternating Laser EXcitation (ALEX)?
- How does a confocal smFRET instrument work?
- What does smFRET data look like?
- What are the key considerations for researchers?
- What are the applications of smFRET?
- What is accurate FRET correction and why does it matter?
Want to know more about bringing dynamic single-molecule techniques to your benchtop?
We believe in making innovative techniques, such as freely diffusing smFRET, accessible to all researchers, regardless of their experience or infrastructure.
Our benchtop tools:
- Are incredibly easy to use – no intensive training or years of expertise required, no complicated sample prep or instrument alignment needed, and results produced within minutes
- Are compact enough to use on your bench top, in ambient light – no darkroom, optical table or laser safe room required, fitting easily into your current infrastructure
- Offer simple workflows that can be implemented with minimal changes to existing workflows
- Come with highly easy-to-use acquisition and analysis software that combines over 50 years of experience, bringing a ‘professor in a box’ into your lab
- Publication-ready data in minutes
