Curious about single-molecule fluorescence techniques such as freely diffusing smFRET and FCS? Our ebook showcases real-world case studies from leading scientists, revealing how methods like these are transforming structural biology, biophysics, and drug discovery.

🔬 Cutting-edge science made accessible
The ebook translates technical advances in smFRET and FCS into practical knowledge you can apply in your own lab.

🌍 Expert voices, global perspectives
Contributions from leaders at UCLA, LMU Munich, University of Pennsylvania, Warwick, Nottingham, and more.

⚡ Actionable insights for your research
Learn how to set up solution-based assays, troubleshoot experiments, and design studies that reveal otherwise hidden dynamics.

🚀 Stay ahead of the curve
Discover emerging trends like three-colour smFRET, live-cell pharmacology, and AI-driven analysis shaping the future of molecular biophysics.

What’s inside?

This collection brings together expert-led seminars and research highlights from pioneers in single-molecule fluorescence. Each chapter demonstrates how smFRET and FCS are applied to unravel molecular mechanisms one molecule at a time.

You’ll learn about:

• The evolution of smFRET – from its earliest experiments to today’s high-throughput, solution-based applications
• Practical assay design –- how to design, benchmark, and analyse robust smFRET experiments for proteins and nucleic acids
• Advanced data analysis – open-source pipelines (FRETBursts, H2MM) for extracting quantitative mechanistic insights
• Intrinsically disordered proteins and neurodegeneration – how FCS uncovers the role of tau and alpha-synuclein in disease
• Membrane protein pharmacology – using FCS to decode receptor clustering, ligand binding, and drug effects in live cells
• Virology at the single-molecule level – smFRET assays dissecting SARS-CoV-2 polymerase activity and antiviral mechanisms
• Future frontiers – multicolour smFRET, live-cell applications, and integrative approaches combining FRET, FCS, and structural biology