In this application note, we demonstrate how fluorescence correlation spectroscopy (FCS) can be performed using the EI-FLEX to assess antibody-antigen binding affinities and generate Kd values.
FCCS is an ideal technique for direct quantification of ternary complex formation. See how the EI-FLEX was used to rapidly generate hook plots and derive time-resolved kinetics in a model DNA oligo system.
Discover how smFRET can capture conformational dynamics on the EI-FLEX Pro using a DNA hairpin model system, measuring the influence of salt on opening and closing rates.
