Embracing single-molecule biophysics in cell lysates or serum
Explore the ‘messy middle’ of biophysics, where cell lysates and serum are combined with single-molecule sensitivity to preserve biological context.
Bringing single-molecule clarity to drug discovery pipelines
In traditional drug discovery, we have long been settling for the average. Explore how single-molecule clarity de-risks biopharma pipelines, targets undruggable IDPs, and powers lab-in-the-loop AI discovery.
Application note – Capturing the structural heterogeneity of pH-sensitive DNA nanoswitches
Discover how smFRET on the EI-FLEX reveals pH-driven DNA triplex nanoswitching, identifying the pH values at which full switching occurs, or heterogeneous populations are present.
Application note – Identifying novel dynamic conformational states from crystal structures
In this application note, we explore how smFRET was used on the EI-FLEX to uncover two additional conformational states in the bacterial helicase Rep, expanding on previously defined structures.
Application note – Defining therapeutic mechanisms of action using smFRET and FCS
Explore how smFRET and FCS can reveal the mechanism of action of two SARS-CoV-2 RNA-dependent RNA polymerase (RdRp) inhibitors using a doubly labelled RNA hairpin as a reporter for RNA extension.
From atomic detail to molecular motion: Adding dynamics to structural biology
Static models only tell half the story. Here, we explore the critical importance of capturing biomolecular dynamics and how mapping these movements links structure to true biological function.
Technical note – How to link accurate FRET efficiency measurements to atomic resolution structures
To ensure calculated distances are accurate and relate to the physical positions of the labelled biomolecules of interest, we discuss the importance of data correction and additional modelling in this technical note.
Application note – Resolving the dynamic structural mechanisms of protein-RNA complexes
Dive into the mechanisms of protein-dependent pseudoknot formation, and see how smFRET adds crucial dynamics to structural data.
Application note – Antibody-antigen binding affinity screening using FCS
In this application note, we demonstrate how fluorescence correlation spectroscopy (FCS) can be performed using the EI-FLEX to assess antibody-antigen binding affinities and generate Kd values.
Application note – Directly quantify ternary complex formation in solution
FCCS is an ideal technique for direct quantification of ternary complex formation. See how the EI-FLEX Pro was used to rapidly generate hook plots and derive time-resolved kinetics in a model DNA oligo system.
Application note – Monitoring biomolecular conformational dynamics: A quantitative approach
Discover how smFRET can capture conformational dynamics on the EI-FLEX Pro using a DNA hairpin model system, measuring the influence of salt on opening and closing rates.
Application note – Measuring nanoscale distances with single-molecule FRET
In this application note, we demonstrate how single-molecule FRET can be used to measure nanoscale distances on the EI-FLEX system using a model DNA duplex.