Core facility case study – A conversation with Alex Payne-Dwyer at the University of York
Hear from Alex Payne-Dwyer, Senior Technical Specialist at the University of York, who discusses his experiences of running the EI-FLEX in a core facility.
Democratising advanced biophysics in core facilities
Bringing smFRET, FCS, and FCCS out of the specialist dark room, the EI-FLEX system is democratising advanced biophysics in core facilities by combining single-molecule resolution with simple, code-free workflows that maximise institutional utility.
30 years of smFRET: A chronological journey through the life sciences’ dynamic revolution
As smFRET turns 30, trace its evolution from a ground-breaking physics concept into the democratised, high-throughput benchtop tool that is transforming the life sciences and modern drug discovery.
Application note – Characterisation of cell-free protein production using FCS
Discover a simple, purification-free method to track real-time protein production and quantification by coupling cell-free protein synthesis with an FCS time course over several hours.
Application note – Purification-free smFRET: Utilising a universal protein ladder directly in mammalian cell lysates
In-cell labelling for smFRET and direct analysis of cell lysates have a wide range of benefits. See how this was applied to generate a universal protein ladder for FRET.
Surface vs solution: The benefits of breaking free from immobilisation
Wondering whether your tethered data is revealing true biological insights or immobilisation artefacts? Explore why combining kinetics on the surface with solution-phase single-molecule validation is essential to avoid misleading data.
Technical note – Biomolecular labelling strategies for smFRET
Curious about how to label samples for smFRET experiments? Explore practical considerations and recommended methods in this technical note.
The Structural Biologist’s guide to single-molecule FRET
Unlock deeper structural insights. Discover the benefits of integrating dynamic smFRET with cryo-EM, X-ray crystallography, and NMR data.
Embracing single-molecule biophysics in cell lysates or serum
Explore the ‘messy middle’ of biophysics, where cell lysates and serum are combined with single-molecule sensitivity to preserve biological context.
Bringing single-molecule clarity to drug discovery pipelines
In traditional drug discovery, we have long been settling for the average. Explore how single-molecule clarity de-risks biopharma pipelines, targets undruggable IDPs, and powers lab-in-the-loop AI discovery.
Application note – Capturing the structural heterogeneity of pH-sensitive DNA nanoswitches
Discover how smFRET on the EI-FLEX reveals pH-driven DNA triplex nanoswitching, identifying the pH values at which full switching occurs, or heterogeneous populations are present.
Application note – Identifying novel dynamic conformational states from crystal structures
In this application note, we explore how smFRET was used on the EI-FLEX to uncover two additional conformational states in the bacterial helicase Rep, expanding on previously defined structures.