Application note – Quantifying affinity, cooperativity, and kinetic stability in ternary complexes using FCCS
In exciting new data from Exciting Instruments HQ, see how FCCS performed on the EI-FLEX Pro to quantify affinity, cooperativity, and kinetic stability in ternary complexes using dual HER2-binding antibodies.
Application note – Single-molecule analysis of protein-mediated ssDNA conformational control
Curious to see smFRET data on ssDNA? Here, we show how the EI-FLEX confirmed DNA wrapping around replication protein A, providing consensus on previously conflicting structural data.
Application note – Resolving ligand-induced conformational heterogeneity using single-molecule FRET
Discover how smFRET performed using the EI-FLEX resolved the conformational changes of a highly dynamic heterodimeric protein, Rag GTPase, that binds nucleotides and the serine/threonine protein kinase complex mTORC1.
From mechanism to medicine: How smFRET is revolutionising our understanding of cellular machinery
In this second celebratory article, we explore how smFRET has revolutionised our understanding of biomolecular mechanisms and how this is now being applied to modern therapeutics.
Core facility case study – A conversation with Alex Payne-Dwyer at the University of York
Hear from Alex Payne-Dwyer, Senior Technical Specialist at the University of York, who discusses his experiences of running the EI-FLEX in a core facility.
Democratising advanced biophysics in core facilities
Bringing smFRET, FCS, and FCCS out of the specialist dark room, the EI-FLEX system is democratising advanced biophysics in core facilities by combining single-molecule resolution with simple, code-free workflows that maximise institutional utility.
30 years of smFRET: A chronological journey through the life sciences’ dynamic revolution
As smFRET turns 30, trace its evolution from a ground-breaking physics concept into the democratised, high-throughput benchtop tool that is transforming the life sciences and modern drug discovery.
Application note – Characterisation of cell-free protein production using FCS
Discover a simple, purification-free method to track real-time protein production and quantification by coupling cell-free protein synthesis with an FCS time course over several hours.
Application note – Purification-free smFRET: Utilising a universal protein ladder directly in mammalian cell lysates
In-cell labelling for smFRET and direct analysis of cell lysates have a wide range of benefits. See how this was applied to generate a universal protein ladder for FRET.
Surface vs solution: The benefits of breaking free from immobilisation
Wondering whether your tethered data is revealing true biological insights or immobilisation artefacts? Explore why combining kinetics on the surface with solution-phase single-molecule validation is essential to avoid misleading data.
Technical note – Biomolecular labelling strategies for smFRET
Curious about how to label samples for smFRET experiments? Explore practical considerations and recommended methods in this technical note.
The Structural Biologist’s guide to single-molecule FRET
Unlock deeper structural insights. Discover the benefits of integrating dynamic smFRET with cryo-EM, X-ray crystallography, and NMR data.