Application note – Antibody-antigen binding affinity screening using Fluorescence Correlation Spectroscopy on the EI-FLEX
In this application note, we demonstrate how fluorescence correlation spectroscopy (FCS) can be performed using the EI-FLEX to assess antibody-antigen binding affinities and generate Kd values.
Application note – Quantifying ternary complex formation using fluorescence cross-correlation spectroscopy on the EI-FLEX system
FCCS is an ideal technique for direct quantification of ternary complex formation. See how the EI-FLEX was used to rapidly generate hook plots and derive time-resolved kinetics in a model DNA oligo system.
Application note – Monitoring biomolecular conformational dynamics with the EI-FLEX system: A quantitative smFRET approach
Discover how smFRET can capture conformational dynamics on the EI-FLEX Pro using a DNA hairpin model system, measuring the influence of salt on opening and closing rates.
Application note – Measuring nanoscale distances with single-molecule FRET on the EI-FLEX system
In this application note, we demonstrate how single-molecule FRET can be used to measure nanoscale distances on the EI-FLEX system using a model DNA duplex.
Technical note – Capturing accurate single-molecule FRET measurements on the EI-FLEX platform
In this technical note, we explore several key components of a confocal FRET experiment that ensure single-molecule sensitivity and accurate determination of FRET efficiencies during acquisition and analysis.