Resource Library

Beyond the average: The EI-FLEX Pro makes high-throughput single-molecule assays industry-ready

In this press release, we announce the launch of the EI-FLEX Pro and EI-FLEX Pro HT, bringing automated, high-throughput single-molecule analysis to the benchtop and enabling industry-ready workflows across drug discovery, biotech, and academic research.

Application note – Measuring nanoscale distances with single-molecule FRET on the EI-FLEX system

In this application note, we demonstrate how single-molecule FRET can be used to measure nanoscale distances on the EI-FLEX system using a model DNA duplex.

Technical note – Capturing accurate single-molecule FRET measurements on the EI-FLEX platform

In this technical note, we explore several key components of a confocal FRET experiment that ensure single-molecule sensitivity and accurate determination of FRET efficiencies during acquisition and analysis.

Mastering smFRET: A practical handbook for researchers

Discover everything you need to understand single-molecule FRET and use it to advance your research. Download the handbook now.

Electronic actuation of surface-immobilised, pH-responsive DNA nanoswitches

Discover how EI-FLEX smFRET reveals pH-driven DNA triplex nanoswitching and confirms that dynamic DNA machines retain fast, reversible conformational control even when densely immobilised on electronic surfaces.

The transitional kinetics between open and closed Rep structures can be tuned by salt via two intermediate states

Discover how single-molecule FRET data on the EI-FLEX helped to uncover two previously hidden intermediate conformational states of a bacterial Rep helicase, overcoming surface-induced artefacts to reveal its dynamic mechanism in action.

Mechanistic insights into the activity of SARS-CoV-2 RNA polymerase inhibitors using single-molecule FRET

Discover how smFRET data from EI-FLEX visualises real-time RNA synthesis by SARS-CoV-2 RdRp and reveals how antiviral inhibitors remodel polymerase dynamics to halt viral replication.

Partial wrapping of single-stranded DNA by replication protein A and modulation through phosphorylation

Discover how smFRET data from the EI-FLEX reveals how RPA partially wraps single-stranded DNA and remodels its structure through phosphorylation.

Precision and accuracy of single-molecule FRET measurements

Discover how the landmark Hellenkamp et al. study set the standard for precise, reproducible single-molecule FRET measurements using DNA rulers.

Delineation of a novel assembly intermediate in retroviral integration pathway

EI-FLEX data revealed a transient tetrameric intermediate in retroviral DNA integration.

Global conformation of the Rag GTPase heterodimer governs eukaryotic amino acid sensing

Discover EI-FLEX data revealing amino acid sensing in eukaryotic cells.

Three-colour single molecule FRET

Discover how Prof. Thorsten Hugel uses three-colour smFRET to explore protein structure, dynamics, and molecular machines in real time.

🚀 Now available: our new upgradable, automated system - the EI-FLEX Pro

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